Journal: Neurotrauma Reports

Article Title: Decreases in Dorsal Cervical Spinal Cord White Matter Tract Integrity Are Associated with Elevated Levels of Serum MicroRNA Biomarkers in NCAA Division I Collegiate Football Players

doi: 10.1089/neur.2021.0036

Figure Lengend Snippet: Correlation between microRNA biomarkers and fractional anisotropy of the white matter dorsal cervical spinal cord. miR-505*, miR-30d, miR-362-3p, and miR-92a were significantly and inversely correlated with FA values in the dorsal (posterior) white matter tracts of the cervical spinal cord. Lower FA values (worse connectivity) are associated with higher miRNA levels. Correlations with the other miRNAs were not significant. FA, fractional anisotropy; miR/miRNA, microRNA.

Article Snippet: RNA was isolated using 100 μL of serum using a serum/plasma miRNA isolation kit (Qiagen, Hilden, Germany) as per the manufacturer's recommended protocol.

Techniques:

Journal: Neurotrauma Reports

Article Title: Decreases in Dorsal Cervical Spinal Cord White Matter Tract Integrity Are Associated with Elevated Levels of Serum MicroRNA Biomarkers in NCAA Division I Collegiate Football Players

doi: 10.1089/neur.2021.0036

Figure Lengend Snippet: Box plot comparing of concentrations of miRNAs in controls versus athletes with lower FA values versus athletes with higher FA values in the white matter dorsal (posterior) cervical spinal tracts. There were significant differences ( p < 0.001) in all miRNA when comparing non-athlete controls ( n = 30) versus all athletes, including those with lower FA values (<0.72; n = 10 scans) and athletes with higher FA values (≥0.72; n = 18 scans). There was an inverse relationship between miRNAs and FA values (except for miR-20a) with elevations in miRNAs with lower FA values. When comparing low and high FA values alone (without controls and without multiple comparisons), miR-505* ( p = 0.021), miR-30d ( p = 0.044), and miR-92a ( p = 0.013) were significantly higher in those with lower FA values (FA, <0.72). Box plots represent medians with interquartile ranges. FA, fractional anisotropy; miR/miRNA, microRNA.

Article Snippet: RNA was isolated using 100 μL of serum using a serum/plasma miRNA isolation kit (Qiagen, Hilden, Germany) as per the manufacturer's recommended protocol.

Techniques:

Journal: Neurotrauma Reports

Article Title: Decreases in Dorsal Cervical Spinal Cord White Matter Tract Integrity Are Associated with Elevated Levels of Serum MicroRNA Biomarkers in NCAA Division I Collegiate Football Players

doi: 10.1089/neur.2021.0036

Figure Lengend Snippet: Area under the ROC curve showing the ability of microRNAs (miR-505*, miR-30d, and miR-92a) to predict decreased fractional anisotropy of the dorsal (posterior) white matter tracts of the cervical spinal cord. FA was dichotomized into lower FA values (<0.72; n = 10 scans) and athletes with higher FA values (≥0.72; n = 18 scans). Three miRNAs that had the most significant association with lower FA values in the dorsal white matter tracts included miR-505* with an AUC = 0.75 (0.54–0.96), miR-30d with an AUC = 0.74 (0.52–0.95), and miR-92a with an AUC = 0.75 (0.53–0.98). AUC, area under the curve; CI, confidence interval; FA, fractional anisotropy; miR/miRNA, microRNA; MRI, magnetic resonance imaging; ROC, receiver operating characteristic.

Article Snippet: RNA was isolated using 100 μL of serum using a serum/plasma miRNA isolation kit (Qiagen, Hilden, Germany) as per the manufacturer's recommended protocol.

Techniques: Magnetic Resonance Imaging

Journal: Neurotrauma Reports

Article Title: Decreases in Dorsal Cervical Spinal Cord White Matter Tract Integrity Are Associated with Elevated Levels of Serum MicroRNA Biomarkers in NCAA Division I Collegiate Football Players

doi: 10.1089/neur.2021.0036

Figure Lengend Snippet: Area under the ROC curve showing how FA values of different cervical spinal cord tracts could predict threshold elevations in miRNA biomarkers. To further evaluate the relationship between decreasing FA values (lower white matter integrity) of specific cervical spinal cord tracts in football athletes and miRNA biomarkers, ROC curves were constructed to depict how the FA could predict threshold elevations in miRNA biomarkers. Each miRNA biomarker was dichotomized based on the ROC curve and compared to the FA of different cervical spinal cord tracts/regions. All AUCs presented in the figures are statistically significant ( p < 0.05). AUC, area under the curve; CI, confidence interval; FA, fractional anisotropy; miR/miRNA, microRNA; MRI, magnetic resonance imaging; ROC, receiver operating characteristic; WM, white matter.

Article Snippet: RNA was isolated using 100 μL of serum using a serum/plasma miRNA isolation kit (Qiagen, Hilden, Germany) as per the manufacturer's recommended protocol.

Techniques: Construct, Biomarker Discovery, Magnetic Resonance Imaging

Journal: Non-Coding RNA

Article Title: The Profile of Circulating Blood microRNAs in Outpatients with Vulnerable and Stable Atherosclerotic Plaques: Associations with Cardiovascular Risks

doi: 10.3390/ncrna8040047

Figure Lengend Snippet: List of the miRNA assays used for qPCR.

Article Snippet: MiRNA was isolated from 300 μL of plasma using a NucleoSpin miRNA Plasma kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s guidelines.

Techniques: Sequencing, Control

Journal: Non-Coding RNA

Article Title: The Profile of Circulating Blood microRNAs in Outpatients with Vulnerable and Stable Atherosclerotic Plaques: Associations with Cardiovascular Risks

doi: 10.3390/ncrna8040047

Figure Lengend Snippet: Results of comparison of miRNA relative plasma level in patients with different characteristics of atherosclerotic plaques (nonparametric test for comparison of multiple Kruskal–Wallis groups due to data distribution different from normal). ( A ) miR-143-3p relative levels. * p -value = 0.0457, ** p -value = 0.0046; ( B ) miR-181b-5p relative levels. * p -value = 0.0179.

Article Snippet: MiRNA was isolated from 300 μL of plasma using a NucleoSpin miRNA Plasma kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s guidelines.

Techniques: Comparison, Clinical Proteomics

Journal: Non-Coding RNA

Article Title: The Profile of Circulating Blood microRNAs in Outpatients with Vulnerable and Stable Atherosclerotic Plaques: Associations with Cardiovascular Risks

doi: 10.3390/ncrna8040047

Figure Lengend Snippet: Association of miRNA levels with the cardiovascular risks estimated by ACC/AHA.

Article Snippet: MiRNA was isolated from 300 μL of plasma using a NucleoSpin miRNA Plasma kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s guidelines.

Techniques:

Journal: Children

Article Title: MicroRNA-126-3p/5p and Aortic Stiffness in Patients with Turner Syndrome

doi: 10.3390/children9081109

Figure Lengend Snippet: Abundance level of miR-126-3p and miR-126-5p in Turner Syndrome patients compared to healthy volunteers as determined by RT-qPCR. The mean relative abundance level (2 −ΔCt ) of all Turner Syndrome patients and healthy volunteers. RNAU6B was used as an endogenous control for the normalization of miRNA. Unpaired Student’s t -test and mean ± standard deviation were used to evaluate differences in abundance levels. *** p ≤ 0.001.

Article Snippet: Total RNA, including miRNAs, was purified from blood samples using PAXgeneTM Blood miRNA Kit on the QIAcubeTM robot (Qiagen, Hilden, Germany), and Dnase I treatment (Qiagen) was performed to remove the residual genomic DNA contamination.

Techniques: Quantitative RT-PCR, Control, Standard Deviation

Journal: EBioMedicine

Article Title: Epstein–Barr Virus MicroRNAs are Expressed in Patients with Chronic Lymphocytic Leukemia and Correlate with Overall Survival ☆

doi: 10.1016/j.ebiom.2015.04.018

Figure Lengend Snippet: Small RNA-sequencing data on B-cells from normal individuals and patients with CLL. Both in B-cells from normal individuals (A) and patients with CLL (B), more than 50% of reads that mapped to the viral genomes were attributed to the EBV genome. A total of 21 EBV miRNAs were found with BART9 being the highest expressed (3645 reads) to BART12 and BHRF1-1 the lowest ones (1 read). Reads from 15 viruses other than EBV were found by RNAseq (C).

Article Snippet: Briefly, 10 ng of total RNA was reverse transcribed using the miRNA reverse transcription kit (Life Technologies, Carlsbad, CA) and a specific reverse-transcription stem-loop primer, according to the manufacturer's protocol.

Techniques: RNA Sequencing Assay